Composite

Part:BBa_M36268:Design

Designed by: Diana Gong, Michaela Hinks   Group: Stanford BIOE44 - S11   (2014-10-24)


LasR-N-(3-oxododecanoyl)-homoserine lactone qsc102 sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 360
    Illegal NheI site found at 141
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
    Illegal AgeI site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The device is optimized for E. coli, and a small sequence of "TAT" was entered between the RBS and the LasR gene for ideal transcription conditions


Source

See the sources documented in the individual parts of this device.

References